Turnover of histones in rat liver during growth and development
The turnover of liver histones and DNA was investigated after the administration of L-(/sup 3/H)lysine or (/sup 3/H)thymidine and L-(methyl-/sup 14/C)methionine to newborn rats. The radioactivities of the different liver histone subfractions and the DNA were examined over a 206-day-period. All the regression curves for liver histones followed first order kinetics up to 120 days post-injection. Histone H1 had a half life of only 10 days, while the slightly lysine rich histones (H2A and H2B) and the arginine rich histones (H3 and H4) had half lives of 44 and 90 days, respectively. The regression curve for liver DNA followed a curva-linear function. By the end of the 206-day-period 60-63% of the (/sup 3/H)thymidine initially incorporated into DNA was still present. Histone H1 and the core histones H2A and H2B turned over independently of the DNA strands, while the turnover of histones H3 and H4 could be attributed solely to cellular migration and replacement. The turnover of the methyl groups within histones H3 and H4 did not vary significantly from the turnover of the polypeptide chains. Perfusion of rat livers with L-(methyl-/sup 3/H)methionine in the presence of puromycin revealed that methylation was a late event occurring for up to 2 h in the absence of protein synthesis.
- Research Organization:
- North Dakota Univ. School of Medicine, Grand Forks
- OSTI ID:
- 5333199
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HISTONES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL HALF-LIFE
CARBON 14 COMPOUNDS
DNA
GROWTH
LIVER
METHIONINE
PERFUSED ORGANS
RATS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AZINES
BODY
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DRUGS
GLANDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques