Reduction of EGF receptor levels in human tumor cells transfected with an antisense RNA expression vector

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90367-4· OSTI ID:5533771

Yamada, Hirotomo; Koizumi, Shinji; Kimura, Masami ^[1]; Shimizu, Nobuyoshi ^[2]

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1. National Inst. of Industrial Health, Kawasaki (Japan)
2. Keio Univ., Tokyo (Japan)

An expression vector was constructed from part of pSV2neo with the 3{prime}-ClaI fragment of the epidermal growth factor (EGF) receptor cDNA inserted in an inverted orientation downstream from the human metallothionein (MT) IIa promoter. The human squamous carcinoma cell line NA, which overproduces EGF receptor, was transfected with this vector and selected for resistance to the neomycin derivative G418. One of the stable transfectants had a 90% reduction cell-surface EGF receptor in response to ZnSO{sub 4}. The nascent EGF receptor peptide was also decreased with concurrent induction of MT mRNA. These data suggest that the antisense transcript regulated by the MT promoter inhibits the expression of the endogenous EGF receptor genes. Although no transcripts from the antisense gene were detected, the results indicate that transfection with the antisense vector provides a technique by which to modulate the number of EGF receptors on the cell surface of squamous cell carcinomas.

• OSTI ID: 5533771
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 184:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
GENE RECOMBINATION
GENE REGULATION
GENES
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANE PROTEINS
METALLOPROTEINS
METALLOTHIONEIN
MITOGENS
NEOMYCIN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SKIN
SULFATES
SULFUR COMPOUNDS
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
ZINC COMPOUNDS
ZINC SULFATES