Human. beta. -actin expression vector system directs high-level accumulation of antisense transcripts
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
The authors have constructed a mammalian expression vector consisting of 3 kilobases of the human ..beta..-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3' splice site to a short DNA polylinker sequence containing unique Sal I, HindIII, and BamHI restriction endonuclease sites followed by a simian virus 40 (SV40) polyadenylylation signal. Two derivatives, containing the selection markers obtained from pSV2gpt or pSV2neo, were also generated. They find that the promoter activity of this vector is as great or greater than that of the SV40 early promoter in a variety of human and rodent cells. The vector was used to generate ..gamma..-actin and ..beta..-tubulin antisense transcripts in human fibroblast cell lines. The antisense transcripts accumulate to levels comparable with that of the highly abundant ..gamma..-actin and ..beta..-tubulin mRNAs.
- Research Organization:
- Stanford Univ. School of Medicine, Palo Alto, CA (USA)
- OSTI ID:
- 5242464
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:14; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACTIN
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBROBLASTS
GENE REGULATION
GENE REPRESSORS
GENES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MESSENGER-RNA
METHIONINE
MICE
MUSCLES
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACTIN
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBROBLASTS
GENE REGULATION
GENE REPRESSORS
GENES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MESSENGER-RNA
METHIONINE
MICE
MUSCLES
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES