Epidermal growth factor (EGF) receptor gene transcription
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6771677
The authors have studied in vitro transcription of the human epidermal growth factor (EGF) receptor proto-oncogene using nuclear extracts of A431 human epidermoid carcinoma cells, which overproduce the EGF receptor. With the in vitro system we found that Sp1 and other trans-acting factors bound to the EGF receptor promoter regions and are required for maximal expression. Fractionation showed that a DEAE-Sepharose fraction (BA) contained a novel factor, which specifically stimulated EGF receptor transcription 5- to 10-fold. The molecular mass of the native form of the factor is about 270-kDa based on its migration on Sephacryl S-300. This factor may activate transcription of the proto-oncogene through a weak or indirect interaction with the DNA template.
- Research Organization:
- National Institutes of Health, Bethesda, MD (USA)
- OSTI ID:
- 6771677
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:13; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
GENE MUTATIONS
GENE REPRESSORS
GENES
GROWTH FACTORS
IN VITRO
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
MUTATIONS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ONCOGENES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
GENE MUTATIONS
GENE REPRESSORS
GENES
GROWTH FACTORS
IN VITRO
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
MUTATIONS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ONCOGENES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES