Quantitative fluorographic detection of /sup 3/H and /sup 14/C on two-dimensional thin-layer chromatographic sheets by an ultra-high-sensitivity TV camera system

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90425-2· OSTI ID:5531884

Miwa, M; Matsumoto, M; Tezuka, M; Okada, S; Ohsuka, S; Fujiwake, H

A method for quantitative detection of /sup 3/H and /sup 14/C on thin layers is described. After impregnation of the TLC sheet with 50% 2,5-diphenyloxazole-tetrahydrofuran, quantitative imaging of the distribution of weak beta-ray-emitting isotopes on the chromatogram was carried out at room temperature by using a TV camera system, which consisted of a two-stage microchannel plate image intensifier, a low-lag vidicon, and an image processor. The method is applicable for /sup 14/C- and /sup 3/H-labeled samples on TLC sheets (10 X 10 cm) emitting more than 0.17 and 7.5 Bq/mm2, respectively. The method is rapid and has a dynamic range far greater than that of film.

• Research Organization: Shizuoka College of Pharmacy, Japan
• OSTI ID: 5531884
• Journal Information: Anal. Biochem.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
TRACER TECHNIQUES
CARBON 14 COMPOUNDS
IMAGE PROCESSING
TRITIUM COMPOUNDS
AUTORADIOGRAPHY
LUMINESCENCE
TELEVISION CAMERAS
THIN-LAYER CHROMATOGRAPHY
CAMERAS
CHEMISTRY
CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
PROCESSING
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques