Role of liver endothelium in the binding and uptake of ceruloplasmin: studies with colloidal gold probe
To determine the mode of uptake of ceruloplasmin (CP) by liver, the protein was labeled with colloidal gold and infused into the portal vein. In cold almost all probes bound to the sinusoidal endothelium, and at 37/sup 0/C internalization via a system of coated pits and vesicles occurred. Only rarely did the probe appear to bypass the endothelium, moving to the albuminal side through the gaps between endothelial cells. In the endothelial cytoplasm, the probe was seen in coated vesicles, endosomes, tubules, and large vesicles which may have formed by fusion of endosomes and tubules. Moreover, externalization of the probe to the abluminal side was noted, and this also occurred via a system of coated vesicles. The findings suggest that the uptake of CP in the liver may be primarily a transendothelial phenomenon (transcytosis).
- OSTI ID:
- 5529133
- Journal Information:
- J. Ultrastruct. Res.; (United States), Journal Name: J. Ultrastruct. Res.; (United States) Vol. 2; ISSN JULRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BODY
CERULOPLASMIN
CHEMICAL BONDS
COLLOIDS
COMPLEXES
COPPER COMPLEXES
DIGESTIVE SYSTEM
DISPERSIONS
ENDOTHELIUM
GLANDS
GLOBULINS
GLOBULINS-ALPHA
GOLD ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
MAMMALS
METALLOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOCOLLOIDS
RATS
RODENTS
TISSUES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
UPTAKE
VERTEBRATES