Nuclide imaging of vascular graft-platelet interactions: comparison of indium excess and technetium subtraction techniques

Journal Article · Tue Apr 01 04:00:00 EST 1986 · J. Surg. Res.; (United States)

DOI:https://doi.org/10.1016/0022-4804(86)90204-0· OSTI ID:5528019

Wakefield, T W; Lindblad, B; Graham, L M; Whitehouse, Jr, W M; Ripley, S D; Petry, N A; Spaulding, S A; Burkel, W E; Stanley, J C

Indium-111-labeled platelet adherence to ePTFE thoracoabdominal vascular prostheses in a canine model (n = 10) was quantitated by (1) an indium-111 excess technique, contrasting graft radioactivity to that in a reference region, and (2) a technetium-99m subtraction technique, with radioactivity of circulating platelets eliminated by discounting background blood activity. Variation in graft thrombogenicity was provided by seeding six prostheses with enzymatically derived autologous endothelial cells, and implanting four prostheses without seeding. Grafts were imaged at 1, 4, and 6 weeks postimplantation, with platelet labeling using indium-111-oxine and red blood cell labeling using technetium-99m. At 7 weeks grafts were excised and gamma activity was measured in proximal, middle, and distal segments. Luminal generation of TxB2 and 6-keto-PGF1 alpha from midportions of grafts was assayed. Indium-111 excess ratios at 6 weeks correlated with actual gamma activity of excised grafts (proximal r = 0.80, P less than 0.01; middle r = 0.73, P less than 0.05; distal r = 0.48, ns) but such a correlation did not exist for the technetium-99m subtraction technique (r = -0.05, -0.25, and 0.16, in the three segments, respectively, all ns). The ratio of graft to aortic TxB2 production revealed a positive correlation with graft gamma activity (r = 0.87, P less than 0.01), and the ratio of graft 6-keto-PGF1 alpha to TxB2 production also correlated with gamma counts (r = -0.64, P = 0.05). In this experimental setting technetium-99m subtraction analysis was an imprecise method of detecting graft platelet accumulation, whereas indium-111 excess ratios proved to be a more accurate method of quantitating vascular prosthetic thrombogenicity.

• Research Organization: Univ. of Michigan Medical School, Ann Arbor
• OSTI ID: 5528019
• Journal Information: J. Surg. Res.; (United States), Journal Name: J. Surg. Res.; (United States) Vol. 4; ISSN JSGRA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADHESION
ANIMAL TISSUES
ANIMALS
AORTA
AROMATICS
ARTERIES
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DOGS
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
FLUORINATED ALIPHATIC HYDROCARBONS
GRAFTS
HALOGENATED ALIPHATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HOURS LIVING RADIOISOTOPES
HYDROXY COMPOUNDS
IMAGE PROCESSING
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC POLYMERS
ORGANS
OXINE
POLYETHYLENES
POLYMERS
POLYOLEFINS
POLYTETRAFLUOROETHYLENE
PROCESSING
PYRIDINES
QUINOLINES
RADIOISOTOPES
SURFACE PROPERTIES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUES
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES