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Regulation of intracellular iron distribution in K562 human erythroleukemia cells

Journal Article · · J. Biol. Chem.; (United States)
OSTI ID:5527757
; ; ; ;
Following a pulse with 59Fe-transferrin, K562 erythroleukemia cells incorporate a significant amount of 59Fe into ferritin. Conditions or manipulations which alter the supply of iron to cells result in changes in the rate of ferritin biosynthesis with consequent variations in the size of the ferritin pool. Overnight exposure to iron donors such as diferric transferrin or hemin increases the ferritin level 2-4- or 6-8-fold above that of the control, respectively. Treatment with the anti-human transferrin receptor antibody, OKT9 (which reduces the iron uptake by decreasing the number of transferrin receptors) lowers the ferritin level by approximately 70-80% with respect to the control. The fraction of total cell-associated 59Fe (given as a pulse via transferrin) that becomes ferritin bound is proportional to the actual ferritin level and is independent of the instantaneous amount of iron taken up. This has allowed us to establish a curve that correlates different levels of intracellular ferritin with corresponding percentages of incoming iron delivered to ferritin. Iron released from transferrin appears to distribute to ferritin according to a partition function; the entering load going into ferritin is set for a given ferritin level over a wide range of actual amounts of iron delivered.
Research Organization:
National Institute of Child Health and Human Development, Bethesda, MD
OSTI ID:
5527757
Journal Information:
J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 10; ISSN JBCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
COMPLEXES
DAYS LIVING RADIOISOTOPES
DISEASES
ELEMENTS
EVEN-ODD NUCLEI
FERRITIN
GLOBULINS
GLOBULINS-BETA
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
IRON
IRON 59
IRON COMPLEXES
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKEMIA
METABOLISM
METALLOPROTEINS
METALS
MOLECULAR WEIGHT
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
TRACER TECHNIQUES
TRANSFERRIN
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
TUMOR CELLS