Copper's influence on iron metabolism in K562 cells

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5213448

Percival, S S; Armstrong, E ^[1]

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1. Univ. of Florida, Gainesville, (United States)

Copper deficiency is associated with a cellular defect in iron metabolism that results in poor hemoglobin synthesis. In order to determine this mechanisms, K562 cells, a human erythroleukemic cell line, were incubated with 1 mM bethocuproine disulfonic acid (BCS) to produce a copper deficiency or were supplemented with 8 {mu}M copper. Hemoglobin was simultaneously induced in some cells by the addition of 25 {mu}M hemin to the culture medium. Incubation with BCS resulted in a 30 to 40% reduction in intracellular Cu/Zn superoxide dismutase activity while supplementation resulted in a 20 to 50% increase in activity. The authors then examined the effect of these copper manipulations on {sup 59}Fe uptake from transferrin, on ferritin levels and on hemoglobin levels. Hemoglobin was only slightly affected by the copper treatments. In both noninduced cells and induced cells, copper supplementation resulted in a greater level of intracellular iron, a greater level of immunoreactive ferritin, and an enhanced uptake of {sup 59}Fe from transferrin. In BCS-incubated cells, intracellular iron, ferritin and {sup 59}Fe uptake from transferrin were reduced by at least 50%. Because the ferritin levels were reduced, intracellular iron mobilization did not appear to be impaired in copper deficiency. The results suggest that copper deficiency impairs the transport of iron by transferrin into the cell.

• OSTI ID: 5213448
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
COMPLEXES
COPPER
DAYS LIVING RADIOISOTOPES
DISEASES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FERRITIN
GLOBINS
GLOBULINS
GLOBULINS-BETA
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMMUNE SYSTEM DISEASES
INHIBITION
INTERMEDIATE MASS NUCLEI
IRON
IRON 59
IRON COMPLEXES
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKEMIA
MAMMALS
MAN
MEMBRANE TRANSPORT
METABOLISM
METALLOPROTEINS
METALS
NEOPLASMS
NUCLEI
NUTRITIONAL DEFICIENCY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
RADIOISOTOPES
SUPEROXIDE DISMUTASE
TRACER TECHNIQUES
TRANSFERRIN
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
TUMOR CELLS
VERTEBRATES