Growth-stimulating effect on transferrin on a hybridoma cell line: Relation to transferrin iron-transporting function

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90241-3· OSTI ID:5714736

Kovar, J; Franek, F ^[1]

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1. Czechoslovak Academy of Sciences, Prague (Czechoslovakia)

The relation of the growth-stimulating capacity of transferrin to its iron-transporting function was investigated in mouse hybridoma PLV-01 cells cultivated in a chemically defined medium. The cells were precultivated in protein-free medium supplemented either with ferric citrate (cells with a high intracellular iron level) or with iron-saturated transferrin (cells with a low intracellular iron level). Iron uptake was monitored after the application of {sup 59}Fe-labeled ferric citrate or pig transferrin. Cultivation of the cells at the optimum growth-stimulating concentration (500{mu}M) of ferric citrate resulted in an intracellular iron level about 100-fold higher than that of cells cultivated at the optimum transferrin concentration. Replacement of pig transferrin with bovine transferrin resulted in similar intracellular iron levels, but the growth-stimulating effect of bovine transferrin was more than one order of magnitude lower. Cells with a high intracellular iron level grew equally well when cultivated with iron-saturated transferrin or with apotransferrin + deferoxamine. The results suggest that transferrin can act as a cell growth factor only in the iron-saturated form. However, several findings of this work indicate that supplying cells with iron cannot be accepted as the full explanation of the transferrin growth-stimulating effect.

• OSTI ID: 5714736
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 182:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOXYLIC ACID SALTS
CATTLE
CHLORIDES
CHLORINE COMPOUNDS
CITRATES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
HALIDES
HALOGEN COMPOUNDS
HYBRIDOMAS
INTERMEDIATE MASS NUCLEI
IRON 59
IRON CHLORIDES
IRON COMPOUNDS
IRON ISOTOPES
ISOTOPES
MAMMALS
METALLOPROTEINS
MICE
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
RUMINANTS
SWINE
TRANSFERRIN
TRANSITION ELEMENT COMPOUNDS
UPTAKE
VERTEBRATES