Multiple post-transcriptional regulatory mechanisms in ferritin gene expression

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.6.1801· OSTI ID:5085066

Mattia, E; Den Blaauwen, J; Van Renswoude, J ^[1]; Ashwell, G ^[2]

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1. Univ. of Amsterdam (Netherlands)
2. National Institutes of Health, Bethesda, MD (USA)

The authors have investigated the mechanisms involved in the regulation of ferritin biosynthesis in K562 human erythroleukemia cells during prolonged exposure to iron. They show that, upon addition of hemin (an efficient iron donor) to the cell culture, the rate of ferritin biosynthesis reaches a maximum after a few hours and then decreases. During a 24-hr incubation with the iron donor the concentrations of total ferritin heavy (H) and light (L) subunit mRNAs rise 2- to 5-fold and 2- to 3-fold, respectively, over the control values, while the amount of the protein increases 10- to 30-fold. The hemin-induced increment in ferritin subunit mRNA is not prevented by deferoxamine, suggesting that it is not directly mediated by chelatable iron. In vitro nuclear transcription analyses performed on nuclei isolated from control cells and cells grown in the presence of hemin indicate that the rates of synthesis of H- and L-subunit mRNAs remain constant. They conclude that iron-induced ferritin biosynthesis is governed by multiple post-transcriptional regulatory mechanisms. They propose that exposure of cells to iron leads to stabilization of ferritin mRNAs, in addition to activation and translation of stored H-and L-subunit mRNAs.

• OSTI ID: 5085066
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:6; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHELATING AGENTS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DEFEROXAMINE
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
EVEN-ODD NUCLEI
FERRITIN
HEME
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMMUNE SYSTEM DISEASES
IMMUNOASSAY
IMMUNOLOGY
IN VITRO
IRON COMPLEXES
IRON COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKEMIA
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MESSENGER-RNA
METALLOPROTEINS
METHIONINE
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RNA
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRANSCRIPTION
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENT COMPOUNDS
TUMOR CELLS
VERTEBRATES