Some factors affecting the lipid secretory phase of fat absorption by intestine in vitro from golden hamster

Journal Article · Thu Jan 01 04:00:00 EST 1981 · J. Lipid Res.; (United States)

OSTI ID:5527440

Strauss, E W; Jacob, J S

We studied the secretory phase of fat absorption by sacs of the everted intestine in vitro when long chain fatty acid and monoglyceride had been taken up from a physicochemically defined bile salt micellar solution. After uptake, a sac received a supplemental incubation in a saline solution without added lipid. The presence of calcium ion in the medium during supplemental incubation was essential for the production and release of resynthesized triglyceride in the form of chylomicrons. The particles appeared to enter the serosal fluid via the lacteals. The rate of secretion of the lipid varied directly with the concentration of calcium ion in the mucosal fluid in the range 100-900 microM. At a given concentration in the serosal or mucosal fluid, the calcium ion in the latter fluid had the greater effect. The study of additional factors included the original location of the segment in the intestine of the living animal, temperature, use of D2O instead of water, and the effects of Ba/sup 2 +/, Sr/sup 2 +/, Mg/sup 2 +/, and La/sup 3 +/ on lipid secretion.

• OSTI ID: 5527440
• Journal Information: J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 22:1; ISSN JLPRA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
BODY
CALCIUM IONS
CHARGED PARTICLES
DIGESTIVE SYSTEM
DYNAMIC FUNCTION STUDIES
GASTROINTESTINAL TRACT
HEAVY WATER
HYDROGEN COMPOUNDS
IN VITRO
INTESTINAL ABSORPTION
INTESTINES
IONS
LIPIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHYSIOLOGY
SECRETION
UPTAKE
WATER