Absorption of D-[{sup 14}C]-glucose and D-[{sup 3}H]-mannose in everted rat jejunum
Journal Article
·
· Transactions of the American Nuclear Society
OSTI ID:552706
- Univ. of New Mexico, Albuquerque, NM (United States)
Radiolabeled elements are useful in measuring physiologic processes in mammalian tissues. Absorption of D-[{sup 14}C]-glucose is often used as a marker for active transport by the sodium-dependent transmembrane glucose/galactose carrier, SGLT1, present in epithelial tissues. Also, D-[{sup 3}H]-mannose monitors the flux of a passively absorbed hexose through the intestinal wall. Many gastrointestinal absorption studies have employed the everted gut sac method developed by Wilson and Wiseman in 1954. Phloridzin is a glycoside derived from apple trees and an effective inhibitor of the sodium-dependent glucose carrier. It was hypothesized that a kinetic study of radiolabeled hexoses could be successfully applied to a modification of this method and assayed by an inhibitory effect of phloridzin on D-[{sup 14}C]-glucose influx into everted rat jejunum and that glycosides transported by the sodium-dependent glucose transporter would have a similar inhibitory effect on D-[{sup 14}C]-glucose influx.
- OSTI ID:
- 552706
- Report Number(s):
- CONF-971125--
- Journal Information:
- Transactions of the American Nuclear Society, Journal Name: Transactions of the American Nuclear Society Vol. 77; ISSN TANSAO; ISSN 0003-018X
- Country of Publication:
- United States
- Language:
- English
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