Acute exposure of rabbit jejunum to ethanol. In vitro uptake of hexoses

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Dig. Dis. Sci.; (United States)

DOI:https://doi.org/10.1007/BF01296262· OSTI ID:6964410

Thomson, A B

The effect of acute exposure of rabbit jejunum to ethanol on the uptake of three hexoses was examined in vitro. With ethanol present in the preincubation medium for 30 min, or directly in the incubation medium for 6 min, glucose uptake was reduced. Kinetic analysis demonstrated that ethanol in the preincubation medium was associated with a rise in the value of the apparent Michaelis constant (Km*), whereas the inhibition of glucose uptake observed with ethanol present directly in the incubation medium was associated with a reduction in the apparent passive permeability coefficient (Pd*), a reduction in the maximal transport rate (Jdm), and an increase in Km*. When increasing concentrations of ethanol were added to the preincubation or to the incubation medium, there was a reduction in the uptake of both 1 mM and 40 mM glucose, galactose, and 3-O-methyl glucose. The addition of 40 mM galactose or 1 mM phloridzin to 40 mM glucose was associated with a 50% reduction in glucose uptake, but this uptake was not further inhibited by the addition of 6% ethanol (v/v). Similarly, the uptake of 3-O-methyl glucose was inhibited by the addition of 40 mM glucose or galactose but no further reduction in uptake was achieved by adding ethanol. Finally, galactose uptake was inhibited by adding 40 mM glucose or 40 mM 3-O-MG, but the addition of 6% ethanol was associated with no further decline in the uptake of galactose.(ABSTRACT TRUNCATED AT 250 WORDS)

• Research Organization: Division of Gastroenterology, University of Alberta, Edmonton, Canada
• OSTI ID: 6964410
• Journal Information: Dig. Dis. Sci.; (United States), Journal Name: Dig. Dis. Sci.; (United States) Vol. 29:3; ISSN DDSCD
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALCOHOLS
ALDEHYDES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
DIGESTIVE SYSTEM
ETHANOL
GALACTOSE
GASTROINTESTINAL TRACT
GLUCOSE
GLYCOSIDES
HEXOSES
HYDROXY COMPOUNDS
INTESTINAL ABSORPTION
INTESTINES
KETONES
KINETICS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PHLORIZIN
RABBITS
REACTION KINETICS
SACCHARIDES
SMALL INTESTINE
UPTAKE
VERTEBRATES