Effects of 5-thio-d-glucose on hexose transport and metabolism

Miscellaneous · Sun Jan 01 00:00:00 EST 1984

OSTI ID:6974549

Kelley, M J

Using the everted sac technique, the mouse small intestine was found to transport 5-thio-D-glucose (5TG) against a concentration gradient, by a sodium- and energy-dependent, phloridzin- and ouabain-sensitive mechanism. At incubation periods of one hour, 5TG inhibited 3-O-methyl-D-glucose (3MG) and D-galactose transport while enhancing net transport of D-glucose. Addition of 5TG and D-glucose produced a dose-related increase in mucosal tissue water D-glucose concentration with a concomitant decrease in L-lactate production. These results suggest that 5TG decreased intestinal utilization of D-glucose via anaerobic glycolysis to partially account for the increased net transport of D-glucose. However, when incubation periods were less than 45 minutes duration, 5TG inhibited net D-glucose transport without affecting L-lactate production. A time- and dose-dependent inhibition of {sup 14}CO{sub 2} production from (1-{sup 14}C) or (6-{sup 14}C) D-glucose by 5TG was observed in everted rings of mouse intestine. The {sup 14}CO{sub 2} produced from (6-{sup 14}C)-D-glucose was not markedly inhibited until incubations were 45 minutes or longer. This study demonstrated that 5TG inhibits D-glucose utilization in the mouse small intestine, which may contribute to the diabetogenic effect observed in vivo. Addition of 5TG stimulated {sup 14}CO{sub 2} production from (1-{sup 14}C) and (6-{sup 14}C) D-glucose in Ehrlich-Lettre (EL) ascites cells, while failing to affect {sup 14}CO{sub 2} production from the tumor bearing mouse (TBM) intestine at any dose employed. When tumor bearing mice were treated with 5TG, stimulation of the HMS occurred in the tumor cells, while variable effects were demonstrated on the HMS of the TBM intestine.

• Research Organization: Louisville Univ., KY (USA)
• OSTI ID: 6974549
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
MEMBRANE TRANSPORT
GLUCOSE
BIOCHEMISTRY
HEXOSES
SMALL INTESTINE
PHYSIOLOGY
ASCITES TUMOR CELLS
CARBON DIOXIDE
LACTIC ACID
MICE
ALDEHYDES
ANIMAL CELLS
ANIMALS
BODY
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMISTRY
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HYDROXY ACIDS
INTESTINES
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RODENTS
SACCHARIDES
TUMOR CELLS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques