Simplified and efficient labeling of human platelets in plasma using indium-111-2-mercaptopyridine-N-oxide: preparation and evaluation

Journal Article · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:5520098

Thakur, M L; McKenney, S L; Park, C H

A water soluble sodium salt of 2-mercaptopyridine-N-oxide (Merc) was evaluated that quantitatively chelated /sup 111/In at a pH range 0.7 to 7.4, and allowed greater than 80% incorporation of /sup 111/In in platelets in plasma. This was dependent on pH, Merc concentration, and platelet concentration. Platelets were labeled using either preformed (/sup 111/In)Merc or incubating platelets with 2.5 ..mu..g dry Merc first and then with /sup 111/In. The latter method provided a simple kit procedure that labeled platelets with negligible alteration of in vitro aggregability. In dogs, labeled platelets had normal survival (7.5 days), 66% recovery, detected vascular thrombi, and pulmonary emboli by scintigraphy. In the kit procedure, the use of Merc compared favorably to that of oxine and tropolone.

• Research Organization: Thomas Jefferson Univ., Philadelphia, PA
• OSTI ID: 5520098
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLASMA
BLOOD PLATELETS
BODY FLUIDS
CARDIOVASCULAR DISEASES
CHEMICAL PREPARATION
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EMBOLI
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
PH VALUE
PYRIDINES
QUINOLINES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPHARMACEUTICALS
SCINTISCANNING
SYNTHESIS
THROMBOSIS
VASCULAR DISEASES
VERTEBRATES