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Evaluation of indium-111-2-mercaptopyridine-N-oxide for labeling leukocytes in plasma: a kit preparation

Journal Article · · J. Nucl. Med.; (United States)
OSTI ID:5520096
; ;

Pure neutrophils, lymphocytes, and mixed leukocytes have been labeled in vitro with /sup 111/In chelated to a nontoxic, water soluble agent 2-mercaptopyridine-N-oxide (Merc). Cells were labeled in isotonic salt-balanced medium with preformed (/sup 111/In)Merc, or in 0.5 ml autologous plasma by incubation with dry Merc first and then with /sup 111/In. The latter method facilitated a kit procedure that required 2 ..mu..g dry Merc when acid citrate dextrose was used as anticoagulant or 20..mu..g when heparin was used. Labeling efficiency was dependent on cell concentration and pH. Labeled cells accumulated avidly in experimental abscesses. In dogs, the liver uptake of labeled cells was only 18.8% compared to that of 48.5% when cells were labeled with (/sup 111/In) oxine.

Research Organization:
Thomas Jefferson Univ., Philadelphia, PA
OSTI ID:
5520096
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ABSCESSES
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLASMA
BODY
BODY FLUIDS
CHEMICAL PREPARATION
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIVER
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXINE
PATHOLOGICAL CHANGES
PYRIDINES
QUINOLINES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPHARMACEUTICALS
SCINTISCANNING
SYNTHESIS
UPTAKE
VERTEBRATES