Cytometric analysis of shape and DNA content in mammalian sperm

Conference · Mon Oct 10 04:00:00 EDT 1983

OSTI ID:5518994

Gledhill, B L

Male germ cells respond dramatically to a variety of insults and are important reproductive dosimeters. Semen analyses are very useful in studies on the effects of drugs, chemicals, and environmental hazards on testicular function, male fertility and heritable germinal mutations. Sperm were analyzed by flow cytometry and slit-scan flow analysis for injury following the exposure of testes to mutagens. The utility of flow cytometry in genotoxin screening and monitoring of occupational exposure was evaluated. The technique proved valuable in separation of X- and Y-chromosome bearing sperm and the potential applicability of this technique in artificial insemination and a solution, of accurately assessing the DNA content of sperm were evaluated-with reference to determination of X- and Y-chromosome bearing sperm.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5518994
• Report Number(s): UCRL-89917; CONF-8310184-1; ON: DE84001786
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL FLOW SYSTEMS
CHEMICAL ANALYSIS
CHROMOSOMAL ABERRATIONS
DATA
DNA
ELECTROMAGNETIC RADIATION
EVALUATION
EXPERIMENTAL DATA
FERTILIZATION
GAMETES
GERM CELLS
GONADS
HAMSTERS
INFORMATION
IONIZING RADIATIONS
MALE GENITALS
MAMMALS
MICE
MORPHOLOGICAL CHANGES
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
QUANTITATIVE CHEMICAL ANALYSIS
RADIATION EFFECTS
RADIATIONS
RODENTS
SPERMATOZOA
TERATOGENESIS
TESTES
TESTING
VERTEBRATES
X RADIATION