Cytometric analysis of shape and DNA content in mammalian sperm
Male germ cells respond dramatically to a variety of insults and are important reproductive dosimeters. Semen analyses are very useful in studies on the effects of drugs, chemicals, and environmental hazards on testicular function, male fertility and heritable germinal mutations. Sperm were analyzed by flow cytometry and slit-scan flow analysis for injury following the exposure of testes to mutagens. The utility of flow cytometry in genotoxin screening and monitoring of occupational exposure was evaluated. The technique proved valuable in separation of X- and Y-chromosome bearing sperm and the potential applicability of this technique in artificial insemination and a solution, of accurately assessing the DNA content of sperm were evaluated-with reference to determination of X- and Y-chromosome bearing sperm.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5518994
- Report Number(s):
- UCRL-89917; CONF-8310184-1; ON: DE84001786
- Resource Relation:
- Conference: National institute of child health and human development testis workshop, Bethesda, MD, USA, 14 Oct 1983
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELL FLOW SYSTEMS
EVALUATION
DNA
QUANTITATIVE CHEMICAL ANALYSIS
SPERMATOZOA
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMAL ABERRATIONS
MORPHOLOGICAL CHANGES
TESTES
EXPERIMENTAL DATA
FERTILIZATION
HAMSTERS
MICE
TERATOGENESIS
TESTING
X RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BODY
CHEMICAL ANALYSIS
DATA
ELECTROMAGNETIC RADIATION
GAMETES
GERM CELLS
GONADS
INFORMATION
IONIZING RADIATIONS
MALE GENITALS
MAMMALS
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550300 - Cytology