Cytometry of mammalian sperm

Conference · Tue Oct 11 04:00:00 EDT 1983

OSTI ID:5505394

Gledhill, B L

Male germ cells respond dramatically to a variety of insults and are important reproductive dosimeters. Semen analyses are very useful in studies on the effects of drugs, chemicals, and environmental hazards on testicular function, male fertility and heritable germinal mutations. The accessibility of male cells makes them well suited for analytical cytology. We might automate the process of determining sperm morphology but should not do so solely for increased speed. Rather, richer tangible benefits will derive from cytometric evaluation through increased sensitivity, reduced subjectivity, standardization between investigators and laboratories, enhanced archival systems, and the benefits of easily exchanged standardized data. Inroads on the standardization of assays for motility and functional integrity are being made. Flow cytometric analysis of total DNA content of individual sperm is an insensitive means to detect exposure to reproductive toxins because of the small size and low frequency of the DNA content errors. Flow cytometry can be applied to determine the proportions of X- and Y-sperm in semen samples.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5505394
• Report Number(s): UCRL-89918; CONF-8310197-1; ON: DE84001763
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
560152 -- Radiation Effects on Animals-- Animals
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CATTLE
CELL FLOW SYSTEMS
CHEMICAL ANALYSIS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CONDENSED AROMATICS
DNA
DOMESTIC ANIMALS
DRUGS
ELECTROMAGNETIC RADIATION
EVALUATION
GAMETES
GENETIC EFFECTS
GERM CELLS
GONADS
HAMSTERS
HETEROCHROMOSOMES
HISTOLOGICAL TECHNIQUES
HYDROCARBONS
IONIZING RADIATIONS
MALE GENITALS
MAMMALS
MICE
MITOMYCIN
MORPHOLOGICAL CHANGES
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
QUANTITATIVE CHEMICAL ANALYSIS
RABBITS
RADIATION EFFECTS
RADIATIONS
RODENTS
RUMINANTS
SPERMATOZOA
TESTES
VERTEBRATES
X CHROMOSOME
X RADIATION
Y CHROMOSOME