Benzo(a)pyrene carcinogenesis: a biochemical selection mechanism
Separation of the metabolic products of benzo(a)pyrene has been readily accomplished by high-pressure liquid chromatography. This technique is uniquely suited to compounds labile to air and light and for resolving positional isomers of phenolic or other types of oxygenated metabolites of this carcinogen. This procedure has been utilized to separate and compare benzo(a)pyrene activation and detoxification products between rat, mouse, and hamster hepatic microsomes and mouse and hamster embryo cell cultures. While metabolite profiles exhibited the same types of derivatives, marked quantitative variation was observed. Microsomal preparation produced large amounts of non-carcinogenic phenols, while intact cell metabolism favored diol formation. These results are in agreement with re-activation of metabolic diols as substrates for further activation to a more proximate carcinogenic species of benzo(a)pyrene, and caution against extrapolating metabolic results from any single test system to other species or tissues.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5518895
- Report Number(s):
- CONF-7609123-1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
BIOCHEMISTRY
CARCINOGENESIS
CELL CONSTITUENTS
CELL CULTURES
CHEMICAL ACTIVATION
CHEMISTRY
CHROMATOGRAPHY
CONDENSED AROMATICS
EMBRYOS
GLYCOLS
HAMSTERS
HYDROCARBONS
HYDROXY COMPOUNDS
MAMMALS
METABOLISM
METABOLITES
MICE
MICROSOMES
ORGANIC COMPOUNDS
ORGANOIDS
PATHOGENESIS
PHENOLS
RATS
RODENTS
SEPARATION PROCESSES
VERTEBRATES