beta. -xylosidase of Thermomonospora

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260270626· OSTI ID:5518218

Ristroph, D L; Humphrey, A E

This study is concerned with characterizing cell-bound inducible ..beta..-xylosidase produced by a strain of the thermophilic bacterial genus Thermomonospora. A crude preparation of this enzyme recovered from sonicated cells of this organism displayed high activity against paranitrophenyl-..beta..-xylopyranoside over a pH range of 5.5-7.7. The temperature optimum, based on a 30-min assay of activity, at pH 6.5 was 70/sup 0/C. The crude enzyme had a thermal half-life of approximately 1 week at 55/sup 0/C and pH 6.5 Xylose inhibited the enzyme. Values of K/sub m/ and V/sub max/ are estimated from the reaction rate data as 0.82 mM and 8 U/L, respectively.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• DOE Contract Number: AC02-76ET20025
• OSTI ID: 5518218
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
DATA
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GLYCOSYL HYDROLASES
HYDROLASES
INFORMATION
INHIBITION
KINETICS
MICROORGANISMS
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PENTOSES
REACTION KINETICS
SACCHARIDES
STABILITY
TEMPERATURE DEPENDENCE
XYLOSE