Kinetic characterization of the extracellular xylanases of Thermomonospora sp

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260270612· OSTI ID:5569627

Ristroph, D L; Humphrey, A E

This study deals with characterizing the extracellular xylanases produced by a strain of the thermophilic bacterial genus Thermomonospora. Supernatant from centrifuged fermentation broth was used as a crude enzyme preparation. From pH 5.5 to pH 7.7 the temperature optimum based on a 10-min assay of activity was 80/sup 0/C. The crude enzyme had a half-life of approximately 1 month when stored at 55/sup 0/C at pH 6.5. The enzyme produced a mixture of xylose oligomers from xylan, with xylobiose occurring in greatest quantity on a molar basis. Only trace quantities of xylose were produced by this hydrolysis.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• DOE Contract Number: AC02-76ET20025
• OSTI ID: 5569627
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
CARBOHYDRATES
CHEMICAL PROPERTIES
CHEMICAL REACTIONS
DATA
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GLYCOSYL HYDROLASES
HEMICELLULOSE
HYDROLASES
HYDROLYSIS
INFORMATION
LYSIS
MONOSACCHARIDES
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PENTOSES
PH VALUE
PHYSICAL PROPERTIES
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TEMPERATURE DEPENDENCE
XYLANASE
XYLANS
XYLOSE