Salt-induction of betaine aldehyde dehydrogenase mRNA, protein, and enzymatic activity in sugar beet. [Beta vulgaris L]

Conference · Wed May 01 00:00:00 EDT 1991 · Plant Physiology, Supplement; (United States)

OSTI ID:5515999

McCue, K F; Hanson, A D ^[1]

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1. Michigan State Univ. Plant Research Lab., East Lansing (United States)

In Chenopodiaceae such as sugar beet (Beta vulgaris L.), glycine betaine (betaine) accumulates in response to drought or salinity stress and functions in the cytoplasm as a compatible osmolyte. The last enzyme in the biosynthetic pathway, betaine aldehyde dehydrogenase (BADH), increases as much as 4-fold in response to rising salinity in the external medium. This increase is accompanied by an increase in both protein and mRNA levels. The steady state increases in BADH were examined at a series of NaCl concentrations from 100 to 500 mM NaCl. BADH protein levels were examined by native PAGE, and by western blot analysis using antibodies raised against BADH purified from spinach. mRNA levels were examined by northern plot analysis of total RNA isolated from the leaves and hybridized with a sugar beet BADH cDNA clone. The time course for BADH mRNA induction was determined in a salt shock experiment utilizing 400 mM NaCl added to the external growth medium. Disappearance of BADH was examined in a salt relief experiment using plants step-wise salinized to 500 mM NaCl and then returned to 0 mM NaCl.

• OSTI ID: 5515999
• Report Number(s): CONF-9107184-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (United States), Vol. 96:1; Conference: Annual meeting of the American Society of Plant Physiology, Albuquerque, NM (United States), 28 Jul - 1 Aug 1991; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
OXIDOREDUCTASES
GENES
SODIUM CHLORIDES
BIOLOGICAL EFFECTS
SUGAR BEETS
ENZYME ACTIVITY
BETAINE
DNA-CLONING
MESSENGER-RNA
PROTEINS
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
BEETS
CARBOXYLIC ACIDS
CHLORIDES
CHLORINE COMPOUNDS
CLONING
DNA HYBRIDIZATION
DRUGS
ENZYMES
FOOD
HALIDES
HALOGEN COMPOUNDS
HYBRIDIZATION
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
QUATERNARY COMPOUNDS
RNA
SODIUM COMPOUNDS
VEGETABLES
550200* - Biochemistry