Betaine aldehyde dehydrogenase isozymes of spinach

Conference · Tue Apr 01 04:00:00 EST 1986 · Plant Physiol., Suppl.; (United States)

OSTI ID:5098189

Hanson, A D; Weretilnyk, E A; Weigel, P

Betaine is synthesized in spinach chloroplasts via the pathway Choline ..-->.. Betaine Aldehyde ..-->.. Betaine; the second step is catalyzed by betaine aldehyde dehydrogenase (BADH). The subcellular distribution of BADH was determined in leaf protoplast lysates; BADH isozymes were separated by 6-9% native PAGE. The chloroplast stromal fraction contains a single BADH isozyme (number1) that accounts for > 80% of the total protoplast activity; the extrachloroplastic fraction has a minor isozyme (number2) which migrates more slowly than number1. Both isozymes appear specific for betaine aldehyde, are more active with NAD than NADP, and show a ca. 3-fold activity increase in salinized leaves. The phenotype of a natural variant of isozyme number1 suggests that the enzyme is a dimer.

• Research Organization: Michigan State Univ., East Lansing
• DOE Contract Number: AC02-76ER01338
• OSTI ID: 5098189
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 80:4
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
BETAINE
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOXYLIC ACIDS
COENZYMES
DRUGS
ENZYME ACTIVITY
ENZYMES
FOOD
LEAVES
LIPOTROPIC FACTORS
NAD
NADP
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
QUATERNARY COMPOUNDS
SPINACH
SYNTHESIS
VEGETABLES