Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus

Halavaty, Andrei S.; Rich, Rebecca L.; Chen, Chao; Joo, Jeong Chan; Minasov, George; Dubrovska, Ievgeniia; Winsor, James R.; Myszka, David G.; Duban, Mark; Shuvalova, Ludmilla; Yakunin, Alexander F.; Anderson, Wayne F.

doi:10.1107/S1399004715004228

Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus

Journal Article · Sat Apr 25 00:00:00 EDT 2015 · Acta Crystallographica. Section D: Biological Crystallography (Online)

DOI:https://doi.org/10.1107/S1399004715004228· OSTI ID:1233329

Halavaty, Andrei S. ^[1]; Rich, Rebecca L. ^[2]; Chen, Chao ^[3]; Joo, Jeong Chan ^[3]; Minasov, George ^[1]; Dubrovska, Ievgeniia ^[1]; Winsor, James R. ^[1]; Myszka, David G. ^[2]; Duban, Mark ^[4]; Shuvalova, Ludmilla ^[1]; Yakunin, Alexander F. ^[3]; Anderson, Wayne F. ^[1]

Northwestern Univ., Chicago, IL (United States). Dept. of Biochemistry and Molecular Genetics; Center for Structural Genomics of Infectious Diseases (CSGID), Chicago, IL (United States)
Biosensor Tools LLC, Salt Lake City, UT (United States)
Univ. of Toronto, ON (Canada). Dept. of Chemical Engineering and Applied Chemistry
Center for Structural Genomics of Infectious Diseases (CSGID), Chicago, IL (United States)

When exposed to high osmolarity, methicillin-resistant Staphylococcus aureus (MRSA) restores its growth and establishes a new steady state by accumulating the osmoprotectant metabolite betaine. Effective osmoregulation has also been implicated in the acquirement of a profound antibiotic resistance by MRSA. Betaine can be obtained from the bacterial habitat or produced intracellularly from choline via the toxic betaine aldehyde (BA) employing the choline dehydrogenase and betaine aldehyde dehydrogenase (BADH) enzymes. Here, it is shown that the putative betaine aldehyde dehydrogenase SACOL2628 from the early MRSA isolate COL ( SaBADH) utilizes betaine aldehyde as the primary substrate and nicotinamide adenine dinucleotide (NAD⁺) as the cofactor. Surface plasmon resonance experiments revealed that the affinity of NAD⁺, NADH and BA for SaBADH is affected by temperature, pH and buffer composition. Finally, five crystal structures of the wild type and three structures of the Gly234Ser mutant of SaBADH in the apo and holo forms provide details of the molecular mechanisms of activity and substrate specificity/inhibition of this enzyme.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1233329

Journal Information:: Acta Crystallographica. Section D: Biological Crystallography (Online), Journal Name: Acta Crystallographica. Section D: Biological Crystallography (Online) Journal Issue: 5 Vol. 71; ISSN 1399-0047; ISSN ABCRE6

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Role for Glycine Betaine Transport in Vibrio cholerae Osmoadaptation and Biofilm Formation within Microbial Communities Kapfhammer, Dagmar; Karatan, Ece; Pflughoeft, Kathryn J. Applied and Environmental Microbiology, Vol. 71, Issue 7 https://doi.org/10.1128/aem.71.7.3840-3847.2005	journal	July 2005
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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Staphylococcus aureus
betaine aldehyde dehydrogenase
high-throughput approach
infectious diseases
structural genomics

Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus

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