cDNA cloning and analysis of betaine aldehyde dehydrogenase, a salt inducible enzyme in sugar beet

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5726253

McCue, K F; Hanson, A D ^[1]

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1. Michigan State Univ., East Lansing (USA)

Betaine accumulates and serves as a compatible osmolyte in some plants subjected to drought or salinity stress. The last enzyme in the betaine biosynthetic pathway is betaine aldehyde dehydrogenase (BADH). The activity of BADH increases in response to increasing salinity levels. This increase in activity corresponds to an increase in protein detectable by immunoblotting, and to an increase in the translatable BADH mRNA. BADH was cloned from a cDNA library constructed in {lambda}gt10 using poly(A){sup +} RNA from sugar beets salinized to 500 mM NaCl. cDNAs were size selected (>1kb) before ligation into the vector, and the library was screened with a spinach BADH cDNA probe. Three nearly full length clones obtained were confirmed as BADH by their nucleotide and deduced amino acid homology to spinach BADH. Clones averaged 1.8 kb and contained open reading frames of 500 amino acids at 80% identity with spinach BADH. RNA gel blot analysis of poly(A){sup +} RNA indicated that salinization to 500 mM NaCl resulted in a 5-fold increase of BADH mRNA level.

• OSTI ID: 5726253
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540310 -- Environment
Aquatic-- Basic Studies-- (1990-)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BEETS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
DNA
DROUGHTS
ENZYMES
FOOD
KINETICS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
REACTION KINETICS
SUGAR BEETS
VEGETABLES