N,N prime -Dimethylthiourea dioxide formation from N,N prime -dimethylthiourea reflects hydrogen peroxide concentrations in simple biological systems

Journal Article · Sun May 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.10.3422· OSTI ID:5514029

Curtis, W E; Muldrow, M E; Parker, N B; Barkley, R; Linas, S L; Repine, J E ^[1]

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1. Univ. of Colorado Health Sciences Center, Denver (USA)

The authors hypothesized that measurement of a specific product from reaction of N,N{prime}-dimethylthiourea (Me{sub 2}TU) and H{sub 2}O{sub 2} would provide a good indication of the H{sub 2}O{sub 2} scavenging and protection seen after addition of Me{sub 2}TU to biological systems. They found that addition of H{sub 2}O{sub 2} to Me{sub 2}TU yielded a single stable product, Me{sub 2}TU dioxide. Me{sub 2}TU dioxide formation correlated with Me{sub 2}TU consumption as a function of added H{sub 2}O{sub 2} concentration and was prevented by simultaneous addition of catalase (but not boiled catalase), superoxide dismutase, dimethyl sulfoxide, mannitol, or sodium benzoate. Me{sub 2}TU dioxide formation, Me{sub 2}TU consumption, and H{sub 2}O{sub 2} concentration increases occurred in mixtures containing phorbol 12-myristate 13-acetate (PMA) and normal human neutrophils but not in mixtures containing PMA and neutrophils from patients with chronic granulomatous disease or in mixtures containing PMA and normal neutrophils and catalase. Me{sub 2}TU dioxide formation also occurred in isolated rat lungs perfused with Me{sub 2}TU and H{sub 2}O{sub 2} but not in lungs perfused with Me{sub 2}TU and elastase, histamine, or oleic acid. In contrast, Me{sub 2}TU dioxide formation did not occur after exposure of Me{sub 2}TU to {sup 60}Co-generated hydroxyl radical or hypochlorous acid in the presence of catalase. The results indicate that reaction of Me{sub 2}TU with H{sub 2}O{sub 2} selectively forms Me{sub 2}TU may be useful for assessing the presence and significance of H{sub 2}O{sub 2} in biological systems.

• OSTI ID: 5514029
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:10; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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