(14C)deoxyglucose uptake and imaging in malignant melanoma

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Journal of Surgical Research; (United States)

DOI:https://doi.org/10.1016/0022-4804(91)90056-R· OSTI ID:5508610

Kern, K A ^[1]

---

1. Department of Surgery, Hartford Hospital, CT (USA)

Since malignant tumors utilize more glucose than normal tissues, tumor uptake and autoradiographic imaging studies using the 14C-labeled glucose analog 2-deoxyglucose (DG) provide a useful preclinical system to determine if similar human tumors will image in vivo with positron emission tomography (PET) using 18F-labeled DG (FDG-PET). We studied B16 murine melanomas of increasing metastatic potential (F1, low; BL-6, intermediate; F10, high) as a feasibility study to determine the potential for human melanoma imaging using FDG-PET. Male C57BL-6 mice (50 g) were implanted sc with 1-mm3 fragments of B16 melanomas. Fourteen days later mice were injected ip with 1.25 muCi of {sup 14}CDG. Sixty minutes later tumor (T) and gastrocnemius muscle (M) were harvested, solubilized, and counted for {sup 14}CDG dpm/mg to estimate glucose utilization. Autoradiographic imaging was carried out similarly, using 2.0 muCi or {sup 14}CDG with 30-day exposure of T and M tissue sections (20 microns thick) to x-ray film. The uptake of {sup 14}CDG (expressed as dpm/mg; % injected dose/g; and tumor-to-muscle uptake ratios) was 6 to 10 times higher in tumors than in muscle tissue (P less than 0.001). All three melanoma cell lines imaged successfully with {sup 14}CDG autoradiography. Tumor uptake of {sup 14}CDG did not correlate with increasing metastatic potential. The experimental B16 murine melanomas F1, BL-6, and F10 extract glucose at higher rates than muscle tissue, a property necessary for successful PET imaging of cutaneous melanoma. The lack of correlation between glucose extraction and metastatic potential suggests that the demands for glucose during tumor growth and metastasis are not related. This is the first laboratory study to predict that human malignant melanoma will image with FDG-PET.

• OSTI ID: 5508610
• Journal Information: Journal of Surgical Research; (United States), Journal Name: Journal of Surgical Research; (United States) Vol. 50:6; ISSN 0022-4804; ISSN JSGRA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
COMPUTERIZED TOMOGRAPHY
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
EMISSION COMPUTED TOMOGRAPHY
EXPERIMENTAL NEOPLASMS
GLUCOSE
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MELANOMAS
METABOLISM
MICE
MONOSACCHARIDES
MUSCLES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
POSITRON COMPUTED TOMOGRAPHY
RODENTS
SACCHARIDES
SKIN
TOMOGRAPHY
VERTEBRATES