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Poly(ADP-ribose) polymerase inhibitors suppress UV-induced human immunodeficiency virus type 1 gene expression at the posttranscriptional level

Journal Article · · Molecular and Cellular Biology; (United States)
; ;  [1]
  1. Institute of Applied Microbiology, University of Tokyo (Japan)
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Gene expression of human immunodeficiency virus type 1 (HIV-1) is induced not only by trans activation mediated through a gene product (tat) encoded by the virus but also by treatment of virus-carrying cells with DNA-damaging agents such as UV light. Employing an artificially constructed DNA in which the chloramphenicol acetyltransferase gene was placed under the control of the HIV-1 long terminal repeat, we analyzed the induction process in HeLa cells and found that inhibitors of poly(ADP-ribose) polymerase suppressed UV-induced HIV-1 gene expression but not tat-mediated expression. We also found that suppression occurs at the posttranscriptional level. These results indicate that HIV-1 gene expression is activated by at least two different mechanisms, one of which involves poly-ADP ribosylation. A possible new role of poly-ADP ribosylation in the regulation of specific gene expression is also discussed.

OSTI ID:
5506773
Journal Information:
Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:7; ISSN MCEBD; ISSN 0270-7306
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
AIDS VIRUS
ALDEHYDES
AMIDES
ANTICOAGULANTS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
CELL CONSTITUENTS
COUMARINS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
GENE REGULATION
HELA CELLS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
NICOTINAMIDE
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PENTOSES
PHOSPHORUS-GROUP TRANSFERASES
PLASMIDS
POLYMERASES
PYRIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RIBOSE
SACCHARIDES
TRANSCRIPTION
TRANSFERASES
ULTRAVIOLET RADIATION
VIRUSES
VITAMIN B GROUP
VITAMINS