Orthotopic bone transplantation in mice. III. Methods of reducing the immune response and their effect on healing

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Transplantation; (United States)

OSTI ID:5503885

Kliman, M; Halloran, P F; Lee, E; Esses, S; Fortner, P; Langer, F

Various methods of reducing the immune response to allogeneic bone grafts, either by pretreating the graft or by immunosuppressing the recipient, were compared. Tibial grafts from B10.D2 mice, either untreated or pretreated in various ways, were transplanted into B10 recipients. The antibody response was followed and the extent of bone healing at 4 months was assessed. Pretreatment of the graft by X-irradiation, freezing, or by incubation in alloantisera (either anti-H-2 or anti-Ia) reduced or abolished the immunogenicity of the graft. Immunosuppression of the recipient with methotrexate or antilymphocyte serum (ALS) also greatly depressed the antibody response. But when healing was assessed, none of these treatments except ALS improved the delayed healing of the bone allografts. The reason for this failure was probably that X-irradiation, freezing, alloantiserum pretreatment, and methotrexate all interfered with bone healing directly, whereas ALS did not. We conclude that many methods will reduce the immune response to allogeneic bone, but that only ALS will improve the healing of the allogeneic bone. Furthermore, as a corollary to the observation that pretreatment with anti-Ia serum markedly reduced the immunogenicity of bone allografts, we conclude that much of the immunogenicity of bone allografts is attributable to a population of Ia-positive cells.

• OSTI ID: 5503885
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 31:1; ISSN TRPLA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
GRAFT-HOST REACTION
GRAFTS
HEALING
IMMUNE SERUMS
IMMUNOSUPPRESSION
IMMUNOSUPPRESSIVE DRUGS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
ORGANS
RADIATION EFFECTS
RADIATIONS
RECOVERY
RODENTS
SKELETON
SOMATIC CELLS
TRANSPLANTS
VERTEBRATES
X RADIATION