Regeneration of hemopoietic precursor cells in spleen organ cultures from irradiated mice: influence of genotype of cells injected and of the spleen microenvironment

Journal Article · Fri May 01 04:00:00 EDT 1981 · Blood; (United States)

OSTI ID:5502222

von Melchner, H; Lieschke, G J

The regeneration of hemopoietic precursor cells (colony-forming cells, CFC) was monitored in spleen organ cultures from lethally irradiated mice injected with 10(7) normal syngeneic or allogeneic bone marrow cells. The important role of the microenvironment in supporting hemopoiesis was confirmed by the failure of mutant S1/S1d spleens to support CFC regeneration in organ cultures. However, the extent and quality of the CFC regeneration was clearly dependent on the genetic properties of the injected cells. Evidence for this was obtained from the regeneration patterns of various CFC types in organ cultured spleens derived from different mouse donor-recipient strain combinations (CBA/CBA, CBA/C57BL, CBA/BALB/c, C57BL/C57BL, C57BL/CBA, C57BL/BALB/c) that maintained the differences in the bone marrow frequency of various CFC types characteristic of the donor strain.

• OSTI ID: 5502222
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 57:5; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
550400 -- Genetics
551000 -- Physiological Systems
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BLOOD FORMATION
BONE MARROW CELLS
CFU
CONNECTIVE TISSUE CELLS
GENETICS
GENOTYPE
IRRADIATION
MAMMALS
MICE
RADIATION EFFECTS
RADIOSENSITIVITY
RODENTS
SOMATIC CELLS
SPLEEN CELLS
TISSUE CULTURES
VERTEBRATES