Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins

Journal Article · Thu Jun 01 00:00:00 EDT 1978 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.75.6.2933· OSTI ID:7052773

Reisner, Y; Itzicovitch, L; Meshorer, A; Sharon, N

Mouse bone marrow and spleen cells were fractionated with the aid of soybean agglutinin and peanut agglutinin. A test for spleen colony-forming units in the isolated fractions showed that the hemopoietic stem cells are agglutinated by both of these lectins. The capacity of the agglutinated fractions to reconstitute lethally irradiated allogeneic mice was investigated. A sequential fractionation of splenocytes from SWR donors by soybean agglutinin and peanut agglutinin, or a single fractionation by soybean agglutinin of splenocytes from BALB/c donors, afforded a cell fraction that successfully reconstituted lethally irradiated (BALB/c x C57BL/6)F/sub 1/ mice, without complications due to graft-versus-host reaction.

• Research Organization: Weizmann Institute of Science, Rehovot (Israel)
• OSTI ID: 7052773
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 75:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
STEM CELLS
GRAFT-HOST REACTION
INHIBITION
IMMUNE REACTIONS
BIOLOGICAL RADIATION EFFECTS
MICE
IMMUNOSUPPRESSION
SPLEEN CELLS
TRANSPLANTS
AGGLUTININS
COBALT 60
GAMMA RADIATION
LETHAL IRRADIATION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals
551000 - Physiological Systems