Interlaboratory comparison of transformation in Syrian hamster embryo cells with model and coded chemicals
Journal Article
·
· Environ. Mutagen.; (United States)
Three independent laboratories tested eight model and five coded chemicals in the Syrian hamster embryo clonal transformation assay system to establish the intra- and interlaboratory reproducibility of the system and to identify sources of variability. When a common cell pool and the same lot of fetal calf serum were used, the three laboratories obtained consensus on the activity of eight model chemicals; five chemicals (benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene, N-methyl-N'-nitro-N-nitrosoguanidine, nitroquinoline-N-oxide, and lead chromate) induced morphological transformation without exogenous metabolic activation and three (N-2-fluorenylacetamide, pyrene, and anthracene) produced no transformation response. Five coded chemicals (2,6-dichloro p-phenylenediamine, 4,4'-oxydianiline, cinnamyl anthranilate, dichlorvos, and reserpine), representative of environmental chemical classes, but not necessarily strong carcinogens, produced more equivocal responses in this interlaboratory study. Efforts to increase the transformation frequency or to amplify the expression of the transformed phenotype constitute some of the approaches which should be explored in order to overcome these limitations.
- Research Organization:
- Arthur D. Little Inc., Cambridge, MA
- OSTI ID:
- 5499035
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 8:1; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTHRACENE
AROMATICS
BENZOPYRENE
CARCINOGENESIS
CELL TRANSFORMATIONS
CHALCOGENIDES
CHROMATES
CHROMIUM COMPOUNDS
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
EMBRYONIC CELLS
HYDROCARBONS
INTERLABORATORY COMPARISONS
LEAD COMPOUNDS
NITROSO COMPOUNDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PATHOGENESIS
PYRENE
TRANSITION ELEMENT COMPOUNDS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTHRACENE
AROMATICS
BENZOPYRENE
CARCINOGENESIS
CELL TRANSFORMATIONS
CHALCOGENIDES
CHROMATES
CHROMIUM COMPOUNDS
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
EMBRYONIC CELLS
HYDROCARBONS
INTERLABORATORY COMPARISONS
LEAD COMPOUNDS
NITROSO COMPOUNDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PATHOGENESIS
PYRENE
TRANSITION ELEMENT COMPOUNDS