Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of Syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Journal Article · Tue Dec 31 23:00:00 EST 1985 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860080403· OSTI ID:5217516

Schechtman, L M; Hatch, G G; Anderson, T M; Putman, D L; Kouri, R E; Cameron, J W; Nims, R W; Spalding, J W; Tennant, R W; Lubet, R A

The intralaboratory reproducibility of a DNA virus (SA7) transformation enhancement assay was investigated using nine carcinogenic and noncarcinogenic compounds representing a variety of chemical classes. By the use of standardized procedures designed to limit assay variables, replicate assay data were collected in two independent laboratories and analyzed for concurrence. The carcinogens, 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene, and N-methyl-N'-nitro-N-nitrosoguanidine yielded reproducible dose-dependent cytotoxicity and positive transformation effects in all experiments in both laboratories. The carcinogens lead chromate, diethylnitrosamine, 4-nitroquinoline-N-oxide, and 2-acetylaminofluorene demonstrated enhancement of SA7 transformation at two or more dose levels in 40-50% of the assays. The noncarcinogenic structural analogs anthracene and pyrene consistently did not produce positive assay responses when tested at dose levels up to the limits of solubility. Good interlaboratory concurrence was demonstrated for these model compounds in the Syrian hamster embryo cell-SA7 assay.

• Research Organization: Microbiological Associates, Inc., Bethesda, MD
• OSTI ID: 5217516
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 8:4; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENOVIRUS
ANIMAL CELLS
ANIMALS
ANTHRACENE
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
CARCINOGEN SCREENING
CELL TRANSFORMATIONS
CHROMATES
CHROMIUM COMPOUNDS
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
EMBRYONIC CELLS
HAMSTERS
HYDROCARBONS
INTERLABORATORY COMPARISONS
LEAD COMPOUNDS
MAMMALS
MICROORGANISMS
NITROSO COMPOUNDS
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PYRENE
RODENTS
SCREENING
SIMIAN VIRUS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
VIRUSES