Comparative pathology of Prudhoe Bay crude oil and inert shell sealants in chicken embryos

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Fundamental and Applied Toxicology; (USA)

DOI:https://doi.org/10.1016/0272-0590(89)90316-3· OSTI ID:5498574

Couillard, C M; Leighton, F A ^[1]

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1. Univ. of Saskatchewan, Saskatoon (Canada)

Pathological changes and body weights were compared in 9-day-old chicken embryos exposed to Prudhoe Bay Crude Oil (PBCO), pharmaceutical mineral oil, or a sealing film on the eggshell. Embryos exposed to 10 or 20 microliters PBCO developed marked ascites or subcutaneous edema, extensive liver necrosis, dilation of the heart, and cellular casts and mineralization in renal tubules. Wet and dry body weights were normal or above normal in embryos exposed to PBCO. No changes were found in embryos exposed to 10 microliters mineral oil; embryos exposed to 20 microliters mineral oil had slight dilation of the heart. Very few embryos survived in eggs that were wrapped in sealing film. All embryos that did survive had greatly reduced body weight and marked dilation of the heart. Four of these embryos had vacuolation of hepatocytes at the surface of the liver, one had superficial liver necrosis, and one embryo had slight ascites. These results indicate that the mechanism of the toxic action of PBCO on the avian embryo involves more than simple physical sealing of the shell.

• OSTI ID: 5498574
• Journal Information: Fundamental and Applied Toxicology; (USA), Journal Name: Fundamental and Applied Toxicology; (USA) Vol. 13:1; ISSN FAATD; ISSN 0272-0590
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020600* -- Petroleum-- Health & Safety
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANIMALS
BIRDS
BODY
CANADA
CARDIOVASCULAR SYSTEM
CHICKENS
DIGESTIVE SYSTEM
EMBRYOS
ENERGY SOURCES
FOSSIL FUELS
FOWL
FUELS
GLANDS
HEART
HYDROCARBONS
KIDNEYS
LIVER
MINERAL OILS
MUSCLES
MYOCARDIUM
NORTH AMERICA
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
PARAFFIN
PATHOLOGICAL CHANGES
PETROLEUM
POLLUTION
SASKATCHEWAN
TOXICITY
VERTEBRATES
WATER POLLUTION
WAXES