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Effect of selenium deficiency on anti-glutathione peroxidase-antibody reactive protein in rat liver

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5494147
; ;

Selenium (Se) deficiency in rats results in a decline of liver glutathione peroxidase (GSH-Px) activity with time. To study the effect of Se deficiency on GSH-Px protein, weanling rats were fed a Se-deficient (< 0.02 ppm) or Se supplemented (0.2 ppm) torula yeast diet, and sacrificed after 0, 3, 7, 14, 21 and 28 days. GSH-Px activity in liver cytosol was measured using H/sub 2/O/sub 2/. To assay GSH-Px protein, anti-GSH-Px-IgG was produced in rabbits. The antisera precipitated both enzyme activity and /sup 75/Se of /sup 75/Se-GSH-Px. Specificity of the IgG was confirmed by immunoblotting: a specific reaction with GSH-Px subunits in Se adequate cytosol but no reaction with cytosol from long-term Se deficient rats. An ELISA for GSH-Px protein used the anti-GSH-Px-IgG and showed a graded increase in IgG reactivity with increasing GSH-Px activity up to 50 mEU. In +Se rats, GSH-Px activity increased 66% over 28 days and IgG reactivity increased in a similar manner. During Se deficiency, GSH-Px activity declined exponentially to 0 by day 21 with a t/sub 1/2/ of 2.8 days. IgG reactivity also decreased exponentially with a t/sub 1/2/ of 5.2 days, but in contrast to GSH-Px activity, IgG reactivity did not decrease to 0. This experiment shows that GSH-Px protein decreases during Se deficiency, though at a slower rate than activity, and that an inactive GSH-Px is apparently present in liver cytosol in early Se deficiency.

Research Organization:
Univ. of Arizona, Tucson
OSTI ID:
5494147
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550501 -- Metabolism-- Tracer Techniques
560301* -- Chemicals Metabolism &amp; Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
MAMMALS
NUCLEI
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PROTEINS
RABBITS
RADIOISOTOPES
RATS
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
TRACER TECHNIQUES
VERTEBRATES