RNA polymerase activities associated with mirex-induced adaptive liver growth

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5493346

Yarbrough, J D; Grimley, J M

Chromatin-bound poly(d(A-T)) dependent RNA polymerase II and I plus III activities were measured in intact and adrenalectomized mirex-dosed rats. In intact mirex-dosed rats there was a 92% decrease in hepatic chromatin-bound RNA polymerase II activity 36 hrs post mirex dose. Chromatin-bound RNA polymerase I plus II activity increased with time post mirex dose: 70% at 12 hrs; 99% at 36 hrs, and 179% at 48 hrs. Adrenalectomy (ADX) reduced chromatin-bound RNA polymerase II activity by 45%. Contrary to the intact response, there was no change in chromatin-bound polymerase II activity in ADX mirex dosed rats. There was a marked change in the composition of total chromatin-bound RNA polymerases with time following the mirex dose. By 36 hrs post mirex dose, chromatin-bound RNA polymerase I plus III was 98% of the total. The sequence of events that occur in mirex-induced adaptive liver growth include: a 70-80% increase in relative liver weight at 72 hrs; a 48 hr peak in (/sup 3/H)thymidine incorporation into DNA; a significant reduction in chromatin-bound RNA polymerase II activity at 37 hrs; and significant increases in polymerase I plus III activity (from 24-48 hrs post mirex dose).

• Research Organization: Mississippi State Univ., Mississippi State
• OSTI ID: 5493346
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BODY
DIGESTIVE SYSTEM
DNA
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
GLANDS
GROWTH
HETEROCYCLIC COMPOUNDS
INSECTICIDES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PESTICIDES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PYRIMIDINES
RATS
RIBOSIDES
RNA POLYMERASES
RODENTS
THYMIDINE
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES