Characterization of the cytochrome c oxidase in isolated and purified plasma membranes from the cyanobacterium Anacystis nidulans

Journal Article · Tue Apr 04 04:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00433a048· OSTI ID:5478585

Peschek, G A; Wastyn, M; Trnka, M; Molitor, V; Fry, I V; Packer, L ^[1]

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1. Univ. of Vienna (Austria)

Functionally intact plasma membranes were isolated from the cyanobacterium (blue-green alga) Anacystis nidulans through French pressure cell extrusion of lysozyme/EDTA-treated cells, separated from thylakoid membranes by discontinuous sucrose density gradient centrifugation, and purified by repeated recentrifugation. Origin and identity of the chlorophyll-free plasma membrane fraction were confirmed by labeling of intact cells with impermeant protein markers, ({sup 35}S)diazobenzenesulfonate and fluorescamine, prior to membrane isolation. Rates of oxidation of reduced horse heart cytochrome c by purified plasma and thylakoid membranes were 90 and 2 nmol min{sup {minus}1} (mg of protein){sup {minus}1}, respectively. The cytochrome oxidase in isolated plasma membranes was identified as a copper-containing aa{sub 3}-type enzyme from the properties of its redox-active and EDTA-resistant Cu{sup 2+} ESR signal, the characteristic inhibition profile, reduced minus oxidized difference spectra, carbon monoxide difference spectra, photoaction and photodissociation spectra of the CO-inhibited enzyme, and immunological cross-reaction of two subunits of the enzyme with antibodies against subunits I and II, and the holoenzyme, of Paracoccus denitrificans aa{sub 3}-type cytochrome oxidase. The data presented are the first comprehensive evidence for the occurrence of aa{sub 3}-type cytochrome oxidase in the plasma membrane of a cyanobacterium similar to the corresponding mitochondrial enzyme.

• DOE Contract Number: FG03-87ER13736
• OSTI ID: 5478585
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:7; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CELL CONSTITUENTS
CELL MEMBRANES
CHALCOGENIDES
CHEMICAL REACTIONS
CYANOBACTERIA
CYTOCHROME OXIDASE
CYTOCHROMES
DAYS LIVING RADIOISOTOPES
ELECTRON SPIN RESONANCE
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
HAEM DEHYDROGENASES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
ORGANIC COMPOUNDS
OXIDATION
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PIGMENTS
PROTEINS
PURIFICATION
RADIOISOTOPES
RESONANCE
SULFUR 35
SULFUR ISOTOPES