Biosynthesis of a 42-kD polypeptide in the cytoplasmic membrane of the cyanobacterium Anacystis nidulans strain R2 during adaptation to low CO/sub 2/ concentration
When cells of Anacystis nidulans strain R2 grown under high CO/sub 2/ conditions (3%) were transferred to low CO/sub 2/ conditions (0.05%), their ability to accumulate inorganic carbon (C/sub i/) increased up to 8 times. Cytoplasmic membranes (plasmalemma) isolated at various stages of low CO/sub 2/ adaptation were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. There was a marked increase of a 42-kilodalton polypeptide in the cytoplasmic membrane during adaptation; a linear relationship existed between the amount of this polypeptide and the C/sub i/-accumulating capability of the cells. No significant changes were observed during this process in the amount of other polypeptides in the cytoplasmic membranes or in the polypeptide profiles of the thylakoid membranes, cell walls, and soluble fractions. Spectinomycin, an inhibitor of protein biosynthesis, inhibited both the increase of the 42-kilodalton polypeptide and the induction of high C/sub i/-accumulating capability. The incorporation of (/sup 35/S)sulfate into membrane proteins was greatly reduced during low CO/sub 2/ adaptation. Radioautograms of the /sup 35/S-labeled membrane proteins revealed that synthesis of the 42-kilodalton polypeptide in the cytoplasmic membrane was specifically activated during the adaptation, while that of most other proteins was greatly suppressed. These results suggested that the 42-kilodalton polypeptide in the cytoplasmic membrane is involved in the active C/sub i/ transport by A. nidulans strain R2 and its synthesis under low CO/sub 2/ conditions leads to high C/sub i/-transporting activity.
- Research Organization:
- Institute of Physical and Chemical Research (RIKEN), Wako, Japan
- OSTI ID:
- 5681222
- Journal Information:
- Plant Physiol.; (United States), Vol. 80:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Sequencing and modification of the gene encoding the 42-kilodalton protein in the cytoplasmic membrane of Synechococcus PCC 7942
Nucleotide sequence of the gene from the cyanobacterium Anacystis nidulans R2 encoding the Mn-stabilizing protein involved in photosystem II water oxidation
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON DIOXIDE
BIOLOGICAL EFFECTS
CYANOBACTERIA
CARBON DIOXIDE FIXATION
POLYPEPTIDES
BIOSYNTHESIS
ACRYLAMIDE
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ADAPTATION
CARBON
CELL WALL
CHEMICAL ACTIVATION
CYTOPLASM
ELECTROPHORESIS
EXPERIMENTAL DATA
GELS
INHIBITION
LABELLED COMPOUNDS
PHOTOSYNTHETIC MEMBRANES
SODIUM COMPOUNDS
SOLUBILITY
SULFATES
SULFUR 35
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
AMIDES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON COMPOUNDS
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
COLLOIDS
DATA
DAYS LIVING RADIOISOTOPES
DISPERSIONS
ELEMENTS
EVEN-ODD NUCLEI
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANES
MICROORGANISMS
NONMETALS
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
550501* - Metabolism- Tracer Techniques
560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)