Morphological transformation of C3H/10T1/2 CL8 cells by procarcinogens

Journal Article · Thu Dec 31 23:00:00 EST 1981 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860040202· OSTI ID:5474245

Oshiro, Y; Balwierz, P S

In order to increase the sensitivity of the C3H/10T1/2 CL8 (10T1/2) cell transformation system, the chemical exposure period was increased to a total of 6 days (two consecutive 3-day exposures). Using this modified procedure, we transformed 10T1/2 cells with procarcinogens such as aflatoxin B/sub 1/, benz(a)anthracene, and 4-nitroquinoline-1-oxide which have been negative in the standard 10T1/2 cell transformation assay. However, ..beta..-naphthylamine was inconclusive and 2-acetylaminofluorine was negative in this modified assay system. Results demonstrate that a simple modification of the 10T1/2 cell transformation method can increase the sensitivity to some procarcinogens that require metabolic activation.

• Research Organization: G.D. Searle and Co., Skokie, IL
• OSTI ID: 5474245
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4:2; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANIMAL CELLS
AROMATICS
AZINES
BENZANTHRACENE
CARCINOGEN SCREENING
CHALCOGENIDES
CONDENSED AROMATICS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
NITRO COMPOUNDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PYRIDINES
QUINOLINES
SCREENING
SENSITIVITY