Morphological transformation of C3H/10T1/2 CL8 cells by procarcinogens. [Mice]
In order to increase the sensitivity of the C3H/10T1/2 CL8(10T 1/2) cell transformation system, we increased the chemical exposure period to a total of 6 days (two consecutive 3-day exposures). Using this modified procedure, we transformed 10T1/2 cells with procarcinogens such as aflatoxin B/sub 1/, benz(a)anthracene, and 4-nitroquinoline-1-oxide which have been negative in the standard 10T1/2 cell transformation assay. However, ..beta..-naphthylamine was inconclusive and 2-acetylaminofluorine was negative in this modified assay system. Our results demonstrate that a simple modification of the 10T1/2 cell transformation method can increase the sensitivity to some procarcinogens that require metabolic activation.
- Research Organization:
- G.D. Searle and Co., Skokie, IL
- OSTI ID:
- 6702818
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4:2; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANIMAL CELLS
AROMATICS
AZINES
BENZANTHRACENE
CARCINOGEN SCREENING
CONDENSED AROMATICS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
METABOLIC ACTIVATION
MORPHOLOGICAL CHANGES
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
QUINOLINES
SCREENING
SENSITIVITY