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Hepatic and extrahepatic uptake of HDL-derived plasma cholesterol in exercised and sedentary rats

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5462522
; ;
The present investigation was designed to study high density lipoprotein (HDL)-derived plasma cholesterol (C) turnover in hepatic and extrahepatic tissues of sedentary (S) and exercised (E) rats. 4-week-old Long Evans rats were exercised for 1 hr, 6 days weekly, for a period of 38 weeks, on a motor-driven treadmill at 0.8 mph at a 12% grade. Animals were injected with HDL that was labelled in vitro with /sup 3/H-cholesteryl ester. Serial blood samples and tissues were collected. HDL-C concentration was lower in E vs S rats (23.0 +/- 1.2 and 26.6 +/- 1.9 mg/dl, p < 0.01). While total plasma C was not different, liver C was higher in S vs E rats (8.2 +/- 0.8 and 7.2 +/- 0.5 mg/g). Adrenal C was higher in E vs S rats (29.5 +/- 2.3 and 20.7 +/- 2.3 mg/g, p < 0.01). Multicompartmental analysis of plasma and tissue tracer response led to development of an 8-component model (5 physiological; 3 nonphysiological) that depicted HDL-derived plasma C turnover. Plasma fraction of tracer declined more rapidly in E vs S rats. E rats cleared nonphysiological tracer more rapidly than S rats, but delayed release of tracer into the plasma longer. Fractional rate of tracer uptake into adrenals, liver, testes, and carcass was greater in E rats. There was a greater fractional turnover rate of tracer in adrenals and liver in S vs E rats. Hence HDL-derived plasma C turnover is altered with vigorous exercise.
Research Organization:
Pennsylvania State Univ., University Park
OSTI ID:
5462522
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BODY
BODY FLUIDS
CHOLESTEROL
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
EXERCISE
GLANDS
GONADS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
LIVER
MALE GENITALS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RATS
RODENTS
STEROIDS
STEROLS
TESTES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES