sup 1 H NMR identification of a. beta. -sheet structure and description of folding topology in putidaredoxin

Journal Article · Tue Apr 23 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00230a007· OSTI ID:5451940

Pochapsky, T C; Ye, Xiao Mei ^[1]

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1. Brandeis Univ., Waltham, MA (USA)

Putidaredoxin (Pdx), a 106-residue globular protein consisting of a single polypeptide chain and a (2Fe-2S) cluster, is the physiological reductant of P-450{sub cam}, which in turn catalyzes the monohydroxylation of camphor by molecular oxygen. No crystal structure has been obtained for Pdx or for any closely homologous protein. The application of two-dimensional {sup 1}H NMR methods to the problem of structure determination in Pdx is reported. A {beta}-sheet consisting of five short strands and one {beta}-turn has been identified from distinctive nuclear Overhauser effect patterns. All of the backbone resonances and a majority of the side-chain resonances corresponding to protons in the {beta}-sheet have been assigned sequence specifically. The sheet contains one parallel and three antiparallel strand orientations. Hydrophobic side chains in the {beta}-sheet face primarily toward the protein interior, except for a group of three valine side chains that are apparently solvent exposed. The potential significance of this hydrophobic patch in terms of biological activity is discussed. The folding topology, as determined by the constraints of the {beta}-sheet, is compared with that of other (2Fe-2S) proteins for which folding topologies are known.

• OSTI ID: 5451940
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:16; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
BARYONS
CARBOXYLIC ACIDS
CONFORMATIONAL CHANGES
ELEMENTARY PARTICLES
FERMIONS
HADRONS
IRON COMPOUNDS
MAGNETIC RESONANCE
METALLOPROTEINS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PROTEINS
PROTONS
PSEUDOMONAS
RESONANCE
SULFUR COMPOUNDS
TRANSITION ELEMENT COMPOUNDS
VALINE