sup 1 H NMR studies of plastocyanin from Scenedesmus obliquus: Complete sequence-specific assignment, secondary structure analysis, and global fold
- Research Institute of Scripps Clinic, La Jolla, CA (USA)
- The University, Liverpool (England)
Two-dimensional {sup 1}H NMR methods have been used to make sequence-specific resonance assignments for the 97 amino acid residues of the plastocyanin from the green alga Scenedesmus obliquus. Assignments were obtained for all backbone protons and the majority of the side-chain protons. Spin system identification relied heavily on the observation of relayed connectivities to the backbone amide proton. Sequence-specific assignments were made by using the sequential assignment procedure. During this process, an extra valine residue was identified that had not been detected in the original amino acid sequence. Elements of regular secondary structure were identified from characteristic NOE connectivities between backbone protons, coupling constant values, and the observation of slowly exchanging amide protons. The protein in solution contains eight {beta}-strands, one short segment of helix, five reverse turns, and five loops. The {beta}-strands may be arranged into two {beta}sheets on the basis of extensive cross-strand NOE connectivities. The chain-folding topology determined from the NMR experiments is that of a Greek key {beta}-barrel and is similar to that observed for French bean plastocyanin in solution and poplar plastocyanin in the crystalline state. While the overall structures are similar, several differences in local structure between the S. obliquus and higher plant plastocyanins have been identified.
- OSTI ID:
- 7193064
- Journal Information:
- Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 27:20; ISSN 0006-2960; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ALGAE
AMINO ACID SEQUENCE
AMINO ACIDS
BARYONS
CARBOXYLIC ACIDS
CHEMICAL SHIFT
CHLOROPHYCOTA
CONFORMATIONAL CHANGES
ELEMENTARY PARTICLES
FERMIONS
HADRONS
MAGNETIC RESONANCE
METALLOPROTEINS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PLANTS
PROTEINS
PROTONS
RESONANCE
SCENEDESMUS
UNICELLULAR ALGAE
VALINE