Sequence-specific sup 1 H NMR assignments and determination of the secondary structure for the activation domain isolated from pancreatic procarboxypeptidase B

Journal Article · Tue Aug 14 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00484a600· OSTI ID:5935263

Vendrell, J; Wider, G; Wuethrich, K ^[1]; Aviles, F X ^[2]

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1. Eidgenoessische Technische Hochschule-Hoenggerberg, Zuerich (Switzerland)
2. Universitat Autonoma de Barcelona (Spain)

Nearly complete sequence-specific {sup 1}H NMR assignments are presented for amino acid residues 3-81 in the 81-residue globular activation domain of porcine pancreatic procarboxypeptidase B isolated after limited tryptic proteolysis of the zymogen. These resonance assignments are consistent with the chemically determined amino acid sequence. Regular secondary structure elements were identified from nuclear Overhauser effects and the sequence locations of slowly exchanging backbone amide protons. The molecule contains two {alpha}-helices, including residues 20-30 and approximately residues 58-72, and a three-stranded antiparallel {beta}-sheet with the individual strands extending approximately from 12 to 17, 50 to 55, and 75 to 77. The identification of these secondary structures and a preliminary analysis of additional long-range NOE distance constraints show that isolated activation domain B forms a stable structure with the typical traits of a globular protein. The data presented here are the basis for the determination of the complete three-dimensional structure of activation domain B, which is currently in progress.

• OSTI ID: 5935263
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:32; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AQUEOUS SOLUTIONS
BARYONS
CARBOXYPEPTIDASES
CHEMICAL SHIFT
DISPERSIONS
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
HADRONS
HEAVY WATER
HYDROGEN COMPOUNDS
HYDROLASES
MAGNETIC RESONANCE
MIXTURES
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PH VALUE
PROTONS
RESONANCE
SOLUTIONS
WATER