Sequential sup 1 H NMR assignments and secondary structure of aponeocarzinostatin in solution

Journal Article · Tue Sep 11 04:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00488a029· OSTI ID:5920449

Remerowski, M L; Glaser, S J; Drobny, G P; Sieker, L C ^[1]; Samy, T S.A. ^[2]

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1. Univ. of Washington, Seattle (USA)
2. Univ. of Miami, FL (USA)

Sequential assignments and secondary structural analysis have been accomplished for the 113-residue apoprotein of the antitumor drug neocarzinostatin (NCS) from Streptomyces carzinostaticus. A total of 98% of the main-chain and 77% of the side-chain resonances have been sequence specifically assigned by use of information from coherence transfer experiments and by sequential and interstrand NOEs. Because of the complexity of the NCS spectrum, several sequential assignments strategies were employed to complete the analysis. Apo-NCS consists of three antiparallel {beta}-sheeted domains by NMR analysis. There is an extensive four-strand antiparallel {beta}-sheet, and two two-stranded domains. One of the two-strand domains is contiguous, S72-N87, with chain reversal occurring through the region L77-R82. The other two-stranded domain has the section G16-A24 antiparallel with respect to the region S62-R70. This secondary structure is consistent with the crystal structure of holo-NCS at 2.8-{angstrom} resolution.

• OSTI ID: 5920449
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:36; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
AQUEOUS SOLUTIONS
BACTERIA
BARYONS
CHROMATOGRAPHY
DISPERSIONS
DRUGS
ELEMENTARY PARTICLES
FERMIONS
GAS CHROMATOGRAPHY
HADRONS
MAGNETIC RESONANCE
MASS SPECTROSCOPY
MICROORGANISMS
MIXTURES
NEOCARCINOSTATIN
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
PHARMACOLOGY
PROTONS
RADIOMIMETIC DRUGS
RESONANCE
SEPARATION PROCESSES
SOLUTIONS
SPECTROSCOPY
STREPTOMYCES