Proton NMR assignments and secondary structure of the snake venom protein echistatin

Journal Article · Tue Dec 17 04:00:00 EST 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00114a004· OSTI ID:5617495

Chen, Yuan; Baum, J ^[1]; Pitzenberger, S M; Garsky, V M; Lumma, P K; Sanyal, G ^[2]

---

1. Rutgers-the State Univ., Piscataway, NJ (United States)
2. Merck Sharp and Dohme Research Labs., West Point, PA (United States)

The snake venom protein echistatin is a potent inhibitor of platelet aggregation. The inhibitory properties of echistatin have been attributed to the Arg-Gly-Asp sequence at residues 24-26. In this paper, sequence-specific nuclear magnetic resonance assignments are presented for the proton resonances of echistatin in water. The single-chain protein contains 49 amino acids and 4 cystine bridges. All of the backbone amide, C{sub alpha}H, and side-chain resonances, except for the {eta}-NH of the arginines, have been assigned. The secondary structure of the protein was characterized from the pattern of nuclear Overhauser enhancements, from the identification of slowly exchanging amide protons, from {sup 3}J{sub c{alpha}H-NH} coupling constants, and from circular dichroism studies. The data suggest that the secondary structure consists of a type I {beta}-turn, a short {beta}-hairpin, and a short-, irregular, antiparallel {beta}-sheet and that the Arg-Gly-Asp sequence is in a flexible loop connecting two strands of the distorted antiparallel {beta}-sheet.

• OSTI ID: 5617495
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:50; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ARGININE
ASPARTIC ACID
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLIC ACIDS
GLYCINE
INHIBITION
MAGNETIC RESONANCE
MATERIALS
NUCLEAR MAGNETIC RESONANCE
ORGANIC ACIDS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
REPTILES
RESONANCE
SNAKES
VENOMS
VERTEBRATES