Calcitonin gene-related peptide stimulates proliferation of human endothelial cells

Journal Article · Tue May 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.9.3299· OSTI ID:5451074

Haegerstrand, A; Larsson, O ^[1]; Dalsgaard, C J ^[2]; Jonzon, B ^[3]; Nilsson, J ^[4]

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1. Karolinska Institute, Stockholm (Sweden)
2. Karolinska Institute, Stockholm (Sweden) Karolinska Hospital, Stockholm (Sweden)
3. Huddinge University Hospital (Sweden)
4. Karolinska Hospital, Stockholm (Sweden)

The effects of the vasoactive perivascular neuropeptides calcitonin gene-related peptide (CGRP), neurokinin A (NKA), neuropeptide Y (NPY), and vasoactive intestinal polypeptide (VIP) on proliferation of cultured human umbilical vein endothelial cells (HUVECs) were investigated. CGRP was shown to increase both cell number and DNA synthesis, whereas NKA, NPY, and VIP were ineffective. {sup 125}I-labeled CGRP was shown to bind to HUVECs and this binding was displaced by addition of unlabeled CGRP, suggesting the existence of specific CGRP receptors. The effect of CGRP on formation of adenosine 3{prime},5{prime}-cyclic monophosphate (cAMP) and inositol phosphates (InsP), two intracellular messengers known to be involved in regulation of cell proliferation, was investigated. CGRP stimulated cAMP formation but was without effect on the formation of InsP. Proliferation, as well as cAMP formation, was also stimulated by cholera toxin. Basic fibroblast growth factor stimulated growth without affecting cAMP or InsP formation, whereas thrombin, which increased InsP formation, did not stimulate proliferation. The authors thus suggest that CGRP may act as a local factor stimulating proliferation of endothelial cells; that the mechanism of action is associated with cAMP formation; and that this effect of CGRP may be important for formation of new vessels during physicological and pathophysiological events such as ischemia, inflammation, and wound healing.

• OSTI ID: 5451074
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:9; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANEMIAS
ANIMAL TISSUES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BLOOD VESSELS
BODY
CALCITONIN
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
GENES
HEALING
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
INFLAMMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISCHEMIA
ISOTOPE APPLICATIONS
ISOTOPES
KININS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PEPTIDE HORMONES
PEPTIDES
PHYSIOLOGY
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECOVERY
RIBOSIDES
SYMPTOMS
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASCULAR DISEASES
VEINS