Mitogenic effects of vasoactive neuropeptides on cultured smooth muscle cell lines

Journal Article · Mon Mar 02 04:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90034-8· OSTI ID:6846916

Mitsuhashi, M; Payan, D G

In order to investigate the relationship between the biochemical pathways that characterize contraction and cell growth, the authors have studied both contraction, mitogenesis and protein synthesis induced by the vasoactive neuropeptides, substance P (SP), calcitonin gene related peptide (CGRP) and vasoactive intestinal polypeptide (VIP) on four different established vascular and non-vascular smooth muscle cell lines. Contraction in vitro was evaluated by light microscopy and recorded photographically. Mitogenesis and protein synthesis were evaluated by (/sup 3/H)-thymidine incorporation into cells and (/sup 3/H)-amino acid incorporation into trichloroacetic acid precipitated materials, respectively. SP stimulated mitogenesis of A7r5 cells (embryonic rat aorta), but failed to induce significant contraction of these cells, whereas, SP induced contraction of cultured adult rat vascular smooth muscle cells (VSMC), but failed to stimulate mitogenesis. CGRP and VIP stimulated mitogenesis and protein synthesis, respectively, of DDT/sub 1/MF-2 cells (hamster vas deferens), but neither induced contraction of this cell line. All three neuropeptides showed no effect on BC/sub 3/H1 (mouse smooth muscle-like) cells. These results suggest that neuropeptides with vasoactive properties modulate different stages of cellular mitogenic responses which may be regulated by the degree of maturation of smooth muscle cell. 22 references, 5 figures.

• Research Organization: Univ. of California Medical Center, San Francisco
• OSTI ID: 6846916
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 40:9; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AORTA
ARTERIES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CONTRACTION
DATA
EMBRYONIC CELLS
EXPERIMENTAL DATA
HAMSTERS
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
MITOGENS
MUSCLES
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES